Anti Nitrite Oxidoreductase (beta-NOR)
Mouse monoclonal antibody
Cat.No. HYB 153-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent:0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Nitrite Oxidoreductase (NOR) is an enzyme catalysing the oxidation of nitrite and is located at the inner side of the cytoplasmic and intracytoplasmic membranes of Nitrobacter (Nb) species (1). NOR from N. hamburgenis (2) consist of at least two major subunits, alpha-NOR and beta-NOR, with molucular masses of approximately1115 kDa and 65 kDa respectively.
The Nitrobacter species are gram-negative microorganism ubiquitos in nature and gain energy from the oxidation of nitrite to nitrate.
Nitrite Oxidoreductase purified from N. hamburgensis.
Beta-subunit of NOR from Nb hamburgensis K4, N. winogradskyi agilis K1, N. winogradskyi 213, N. winigradskyi 215, N. winogradskyi 255, and N. vulgaris K48 (3)
HYB 153-01 reacts with a different epitope compared with HYB 153-07
HYB 153-01 reacts weel in Elisa coated with cell extract as well as immunoblotting with a band of approximately 64 kDa corresponding to the beta-subunit of NOR (3,4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16,000 | 3 |
| Immunoblotting | Yes | 3,4 | |
| Immunohistochemistry | Yes | 4 |
1. Sundermeyer-Klinger, H., Meyer, W., Warninghoff, B. and Bock, E. (1984) Membrane-bound nitrite oxidoreductase of Nitrobacter: evidence for a nitrate reductase system. Arch Microbiol. 140. 153-158.
2. Meincke, M., Bock, E., Kastrau, D. and Kroneck, P.M.H. (1992) Nitrite oxidoreductase from Nitrobacter hamburgensis: redox centers and their catalytic role. Arch. Microbiol. 158, 127-131
3. Aamand, J., Ahl, T., and Spieck, E. (1996) Monoclonal antibodies recognizing nitrite oxidoreductase of Nitrobacter hamburgensis, N. winogradskyi, and N. vulgaris. Appl.Environ.Microbiol. 62, 2352-2355
4. Bartosch, S., Wolgast, I., Spieck, E. and Bock, E. (1990) Identification of nitrite-oxidizing bacteria with monoclonal antibodies recognizing the nitrite oxidoreductase. Appl.Environ.Microbiol. 65, 4126-4133.
